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D9781

Sigma-Aldrich

Anti-DNP antibody produced in goat

whole antiserum

Synonym(s):

Anti-Dinitrophenol

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:8,000
quantitative precipitin assay: 1.6-2.4 mg/mL

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-dinitrophenyl (DNP) antibody is produced in goat using DNP-BSA as the immunogen. The antiserum has been treated to remove lipoproteins. The product is determined to be reactive with DNP-BSA and DNP-RSA but not BSA (bovine serum albumin) or RSA (rabbit serum albumin) by immunoelectrophoresis (IEP).

Immunogen

A DNP-BSA conjugate

Application

Anti-DNP antibody produced in goat has been used in DNA immunoFISH and for differential staining of blastocysts.
Anti-DNP antibody produced in goat was used to study the effects of IgE on the clustering of FcεRI by time-resolved phosphorescence anisotropy.

Preparation Note

delipidized

Analysis Note

Antiserum is tested for specificity and titer by immunoelectrophoresis and quantitative precipitin techniques.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mei-Ling Yang et al.
Diabetes, 71(9), 1979-1993 (2022-06-23)
Inflammation and oxidative stress in pancreatic islets amplify the appearance of various posttranslational modifications to self-proteins. In this study, we identified a select group of carbonylated islet proteins arising before the onset of hyperglycemia in NOD mice. Of interest, we
Jinming Song et al.
Biochemistry, 41(3), 881-889 (2002-01-16)
Rat mucosal-type mast cells of the RBL-2H3 line express a glycoprotein termed the MAst cell Function-associated Antigen (MAFA). When MAFA is clustered by its specific monoclonal antibody G63, secretion normally triggered by aggregating these cells' type I Fcepsilon receptor (FcepsilonRI)
Miguel A Velazquez et al.
Biochimica et biophysica acta, 1864(2), 590-600 (2017-12-03)
Mouse maternal low protein diet exclusively during preimplantation development (Emb-LPD) is sufficient to programme altered growth and cardiovascular dysfunction in offspring. Here, we use an in vitro model comprising preimplantation culture in medium depleted in insulin and branched-chain amino acids
M A Velazquez et al.
Human reproduction (Oxford, England), 31(9), 1970-1980 (2016-07-13)
Does advanced maternal age (AMA) in mice affect cardiometabolic health during post-natal life in offspring derived from an assisted reproduction technology (ART) procedure? Offspring derived from blastocysts collected from aged female mice displayed impaired body weight gain, blood pressure, glucose
Insulin and branched-chain amino acid depletion during mouse preimplantation embryo culture programmes body weight gain and raised blood pressure during early postnatal life
Velazquez M
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 590-600 (2018)

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