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D199303

Sigma-Aldrich

2,4-Dinitrophenylhydrazine

reagent grade, 97%

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Synonym(s):
(2,4-dinitrophenyl)-hydrazine, DNPH
Linear Formula:
(O2N)2C6H3NHNH2
CAS Number:
Molecular Weight:
198.14
Beilstein/REAXYS Number:
615586
MDL number:
PubChem Substance ID:
NACRES:
NA.21

grade

reagent grade

assay

97%

contains

33% water (minimum)

mp

197-200 °C (lit.)

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

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1 of 4

This Item
D198501325376900022
vibrant-m

D199303

2,4-Dinitrophenylhydrazine

vibrant-m

D198501

2,4-Dinitrophenol

vibrant-m

325376

Bis(2,4-dinitrophenyl) oxalate

grade

reagent grade

grade

-

grade

-

grade

-

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

-

mp

197-200 °C (lit.)

mp

108-112 °C (lit.)

mp

189-190 °C (lit.)

mp

-

contains

33% water (minimum)

contains

≥15% water

contains

-

contains

-

General description

2,4-Dinitrophenylhydrazine (DNPH), a hydrazine derivative, is a potential mutagenic agent. Its molecular and crystal structure has been analyzed. The intra- and intermolecular hydrogen bonds in DNPH have been investigated by vibrational spectroscopic analysis. The mechanism of isothermal degradation of DNPH has been studied by differential scanning calorimetry (DSC).

Application

2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls. It may be used as a colorimetric reagent in the quantitative estimation of prednisone.

Packaging

500G contains total wet product weight of ~485G. Variance is due to addition of water to meet DOT requirements.

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Desen. Expl. 1

supp_hazards

Storage Class

4.1A - Other explosive hazardous materials

wgk_germany

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Kinetic analysis of isothermal decomposition of 2, 4-dinitrophenylhydrazine using differential scanning calorimetry.
Musuc AM, et al.
Thermochimica Acta, 448(2), 130-135 (2006)
2,4-dinitrophenylhydrazine: a sensitive quantitative reagent for determining microgram quantities of prednisone.
A L Woodson
Journal of pharmaceutical sciences, 60(10), 1538-1542 (1971-10-01)
J. Chem. Soc. Pak., 14, 73-73 (1992)
Clare L Hawkins et al.
Free radical biology & medicine, 46(8), 965-988 (2009-05-15)
Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of
Journal of the American Oil Chemists' Society, 70, 881-881 (1993)

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