11093070910
Roche
Biotin-16-dUTP
Synonym(s):
Bio-16-dUTP tetralithium salt, Biotin-labeled Uridines, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′-deoxyuridine 5′-triphosphate
About This Item
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form
solution
Quality Level
packaging
pkg of 50 μL (50 nmol; 1mM)
manufacturer/tradename
Roche
color
colorless
solubility
water: miscible
storage temp.
−20°C
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General description
Application
- For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation. Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
- as a substrate for terminal transferase, DNA polymerase I (holoenzyme and Klenow fragment), Taq DNA polymerase, and reverse transcriptase (e.g., Transcriptor). The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling
- In fluorescence in situ hybridization (FISH)
Biochem/physiol Actions
Quality
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).
Analysis Note
Emax241: E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.
Other Notes
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
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