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11093070910

Roche

Biotin-16-dUTP

Synonym(s):

Bio-16-dUTP tetralithium salt, Biotin-labeled Uridines, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′-deoxyuridine 5′-triphosphate

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About This Item

Empirical Formula (Hill Notation):
C32H52N7O18P3S
CAS Number:
Molecular Weight:
947.78
UNSPSC Code:
41116100

form

solution

Quality Level

packaging

pkg of 50 μL (50 nmol; 1mM)

manufacturer/tradename

Roche

color

colorless

solubility

water: miscible

storage temp.

−20°C

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General description

Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.Biotin-16-dUTP is used to produce biotinylated DNA probes that are commonly used to detect specific DNA and RNA sequences in different methods like Southern and Northern blotting, in situ hybridization, and sandwich assays.

Application

Biotin-16-dUTP has been used:
  • For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation. Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
  • as a substrate for terminal transferase, DNA polymerase I (holoenzyme and Klenow fragment), Taq DNA polymerase, and reverse transcriptase (e.g., Transcriptor). The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling
  • In fluorescence in situ hybridization (FISH)

Biochem/physiol Actions

Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.

Quality

Typical analysis: At least 85% deoxynucleoside triphosphate (Biotin-16-dUTP) (HPLC, area%).
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).

Analysis Note

Absorption: The spectral data of Biotin-16-dUTP (at pH 7.3):
Emax241: E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.

Other Notes

Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate- Biotin Luminescence Detection Kit.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

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Sudhanshu Agrawal et al.
American journal of physiology. Cell physiology, 311(3), C386-C391 (2016-07-15)
The water-soluble biotin (vitamin B7) is indispensable for normal human health. The vitamin acts as a cofactor for five carboxylases that are critical for fatty acid, glucose, and amino acid metabolism. Biotin deficiency is associated with various diseases, and mice
Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction
DAY PJR
Biochemical Methods, 267, 119-123 (1990)
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Adjuvant Treatment for POLE Proofreading Domain?Mutant Cancers: Sensitivity to Radiotherapy, Chemotherapy, and Nucleoside Analogues
Van Gool IC, et al.
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