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  • Effect of purified fractions from cell culture supernate of high-density pre-B acute lymphoblastic leukemia cells (ALL3) on the growth of ALL3 cells at low density.

Effect of purified fractions from cell culture supernate of high-density pre-B acute lymphoblastic leukemia cells (ALL3) on the growth of ALL3 cells at low density.

Electrophoresis (2016-11-03)
Sapan J Patel, Costel C Darie, Bayard D Clarkson
ABSTRACT

The mechanisms underlying the aberrant growth and interactions between cells are not understood very well. The pre-B acute lymphoblastic leukemia cells directly obtained from an adult patient grow very poorly or do not grow at all at low density (LD), but grow better at high starting cell density (HD). We found that the LD ALL3 cells can be stimulated to grow in the presence of diffusible, soluble factors secreted by ALL3 cells themselves growing at high starting cell density. We then developed a biochemical purification procedure that allowed us to purify the factor(s) with stimulatory activity and analyzed them by nanoliquid chromatography-tandem mass spectrometry (nanoLC-MS/MS). Using nanoLC-MS/MS we have identified several proteins which were further processed using various bioinformatics tools. This resulted in eight protein candidates which might be responsible for the growth activity on non-growing LD ALL3 cells and their involvement in the stimulatory activity are discussed.

MATERIALS
Product Number
Brand
Product Description

SAFC
EX-CELL® 325 PF CHO Serum-Free Medium for CHO Cells, protein free, liquid, sterile-filtered, suitable for cell culture
SAFC
EX-CELL® 302 Serum-Free Medium for CHO Cells, without L-glutamine, without phenol red, without sodium bicarbonate, dry powder, suitable for cell culture