MilliporeSigma
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14340C

SAFC

EX-CELL® 325 PF CHO Serum-Free Medium for CHO Cells

protein free, liquid, sterile-filtered, suitable for cell culture

MDL number:
NACRES:
NA.75

description

for research or for further manufacturing use
without L-glutamine

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

glucose: 3.45 g/L
L-glutamine: no
NaHCO3: 1.6 g/L
HEPES: 7.5 mM
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
14326C24326C24340C
form

liquid

form

liquid

form

dry powder

form

dry powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable

components

glucose: 3.45 g/L
L-glutamine: no
NaHCO3: 1.6 g/L
HEPES: 7.5 mM
phenol red: yes

components

HEPES: yes
phenol red: no
L-glutamine: no
NaHCO3: yes
glucose: yes

components

-

components

-

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

EX-CELL™ 325 PF CHO is a protein-free medium which has been developed for the growth of Chinese Hamster Ovary (CHO) cells. Because it contains no large macromolecules, EX-CELL 325 PF CHO facilitates the isolation and purification of secreted proteins from the cells. CHO cells propagated in EX-CELL 302 serum-free media (Catalog No. 14324C and 14326C) can be transferred directly into this protein-free medium without extensive weaning protocols. This medium is supplied without L-glutamine and does not contain purines or pyrimidines to provide an appropriate medium for specialized CHO cell lines (i.e., Glutamine Synthetase, or the GS System™, and DHFR- selection systems).

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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