MilliporeSigma
All Photos(1)

24340C

SAFC

EX-CELL® 325 PF CHO Serum-Free Medium for CHO Cells

protein free, dry powder, suitable for cell culture

MDL number:
NACRES:
NA.71

description

for research or for further manufacturing use
without L-glutamine

Quality Level

form

dry powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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This Item
24360C24326C14340C
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400

description

for research or for further manufacturing use

description

-

description

-

description

for research or for further manufacturing use, without L-glutamine

General description

EX-CELL® 325 PF CHO is a protein-free medium which has been developed for the growth of Chinese Hamster Ovary (CHO) cells. Because it contains no large macromolecules, EX-CELL 325 PF CHO facilitates the isolation and purification of secreted proteins from the cells. CHO cells propagated in EX-CELL 302 serum-free media can be transferred directly into this protein-free medium without extensive weaning protocols. This medium is supplied without L-glutamine and does not contain purines or pyrimidines to provide an appropriate medium for specialized CHO cell lines (i.e., Glutamine Synthetase, or the GS System and DHFR- selection systems).

Quantity

Formulated to contain 21.0 grams of powder per liter of medium.

Reconstitution

Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
GS System is a trademark of Lonza Group Ltd.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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