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Animal-component free, with HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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CHO Medium
MDL number:

Quality Level


for research or for further manufacturing use






Drug/Device Master File available


cell culture | mammalian: suitable


HEPES: yes
NaHCO3: yes
phenol red: no
L-glutamine: no

shipped in


storage temp.


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Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

Features and Benefits

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity. Cell densities in excess of 8 × 106 cells/ml have been achieved in batch culture systems. Increases in protein productivity up to 500% per cell have been observed.

Other Notes

Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.


Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids




Not applicable


Not applicable

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