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C9098

SAFC

EX-CELL® ACF CHO Medium

Animal-component free, without L-glutamine, use at 21.5 g/L, dry powder, suitable for cell culture

Synonym(s):
CHO Medium
MDL number:
NACRES:
NA.75

Quality Level

description

for research or for further manufacturing use

form

dry powder

quality

Drug/Device Master File available

concentration

21.5 g/L

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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SAFC

SAFC

C9098

EX-CELL® ACF CHO Medium

concentration

21.5 g/L

concentration

-

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

500

description

for research or for further manufacturing use

description

-

description

-

description

-

Features and Benefits

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity.

Other Notes

Proprietary formulation containing inorganic salts, HEPES, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.
Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

Reconstitution

Powdered media are extremely hygroscopic and should be protected from atmospheric moisture. Preparing a concentrated solution of medium is not recommended as precipitates may form.

1. Measure out 90% of final required volume of tissue culture grade water. Water temperature should be 25-40oC.
2. While stirring the water, add the powdered medium (21.5 g/L). Heat may enhance solubility but do not go above 40oC.
3. Rinse original package with a small amount of water to remove all traces of the powder. Add to solution in step 2.
4. Stir until dissolved.
5. Add 0.6g/L (range 0.6-1.2g/L or higher depending on individual cell line) of G3126 L-Glutamine.
6. Add 1.5 g/L of S8875 sodium bicarbonate (If increased buffering capacity is desired this amount can be increased to 3.0 g/L).
7. Adjust the pH to 7.45 range (7.3-7.6) using 5 N NaOH.
8. Use additional water to bring the solution to final volume.
9. Sterilize immediately by filtration using a membrane with a porosity of <0.22 microns. Do not use poly-ether sulfones (PES) type of membranes. We recommend PVDF as the best inert type of filter membrane.
10. Aseptically dispense medium into a sterile container. Store at 2-8 °C in the dark.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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