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C9098
EX-CELL® ACF CHO Medium
Animal-component free, without L-glutamine, use at 21.5 g/L, dry powder, suitable for cell culture
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Quality Level
description
for research or for further manufacturing use
form
dry powder
quality
Drug/Device Master File available
concentration
21.5 g/L
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
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Related Categories
1 of 4
This Item | 24366C | 24360C | 24326C |
|---|---|---|---|
| concentration 21.5 g/L | concentration - | concentration - | concentration - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable (suspension) | technique(s) cell culture | mammalian: suitable (suspension) | technique(s) cell culture | mammalian: suitable (suspension) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 500 |
| description for research or for further manufacturing use | description - | description - | description - |
Features and Benefits
Other Notes
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.
Reconstitution
1. Measure out 90% of final required volume of tissue culture grade water. Water temperature should be 25-40oC.
2. While stirring the water, add the powdered medium (21.5 g/L). Heat may enhance solubility but do not go above 40oC.
3. Rinse original package with a small amount of water to remove all traces of the powder. Add to solution in step 2.
4. Stir until dissolved.
5. Add 0.6g/L (range 0.6-1.2g/L or higher depending on individual cell line) of G3126 L-Glutamine.
6. Add 1.5 g/L of S8875 sodium bicarbonate (If increased buffering capacity is desired this amount can be increased to 3.0 g/L).
7. Adjust the pH to 7.45 range (7.3-7.6) using 5 N NaOH.
8. Use additional water to bring the solution to final volume.
9. Sterilize immediately by filtration using a membrane with a porosity of <0.22 microns. Do not use poly-ether sulfones (PES) type of membranes. We recommend PVDF as the best inert type of filter membrane.
10. Aseptically dispense medium into a sterile container. Store at 2-8 °C in the dark.
Legal Information
supplement
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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