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MilliporeSigma
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Key Documents

C2059

Cloning cylinders, glass

volume 250 μL

Synonym(s):

cloning cylinder, cloning equipment

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About This Item

UNSPSC Code:
41106618
NACRES:
NB.22

material

light brown (tinted)

sterility

sterile; electron beam irradiated

packaging

dish of 10 cylinders

manufacturer/tradename

Millipore TR1005

size

10 mm × 10 mm

volume

250 μL

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General description

Individual colonies of transfected cells can be isolated and picked from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells are then dissociated within the cylinder, resuspeneded, and transferred to a new vessel as a pure colony.
These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.

Packaging

Packaged in 60 mm dish with silicone grease on one end

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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