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  • "Dilute & shoot" approach for rapid determination of trace amounts of nicotine in zero-level e-liquids by reversed phase liquid chromatography and hydrophilic interactions liquid chromatography coupled with tandem mass spectrometry-electrospray ionization.

"Dilute & shoot" approach for rapid determination of trace amounts of nicotine in zero-level e-liquids by reversed phase liquid chromatography and hydrophilic interactions liquid chromatography coupled with tandem mass spectrometry-electrospray ionization.

Journal of chromatography. A (2013-04-04)
Paweł Kubica, Agata Kot-Wasik, Andrzej Wasik, Jacek Namieśnik
ABSTRACT

Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach "dilute & shoot" which makes this important step quick and not complicated. The chromatographic separation was carried out on a Zorbax XDB column (RPLC method) and Ascentis Si column (HILIC mode). Within-run precisions (CVs) measured at three concentration levels were as follows: 0.73%, 0.98% and 1.44% for RPLC method and 1.39%, 1.44% and 0.57% (HILIC mode). Between-run CVs were as follows: 1.94%, 1.02% and 1.22% for RPLC mode and 1.49%, 1.20% and 1.22% for HILIC mode. The detection limits of RPLC and HILIC modes were 4.08 and 3.90 ng/mL respectively. The proposed procedures are rapid, not complicated, sensitive and are suitable for fast determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes.

MATERIALS
Product Number
Brand
Product Description

Supelco
Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
Supelco
Ascentis® Si HPLC Column, 3 μm particle size, L × I.D. 10 cm × 3 mm
Supelco
Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
Supelco
Ascentis® Si HPLC Column, 3 μm particle size, L × I.D. 3 cm × 2.1 mm
Supelco
Ascentis® Si HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm
Supelco
Ascentis® Si HPLC Column, 3 μm particle size, L × I.D. 5 cm × 2.1 mm
Supelco
Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm