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581509-U

Supelco

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 2.1 mm

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eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Quality Level

agency

suitable for USP L3

product line

Ascentis®

feature

endcapped: no

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 2.1 mm

surface area

450 m2/g

impurities

<5 ppm metals

matrix

fully porous particle
silica gel high purity, spherical

matrix active group

silica phase

particle size

5 μm

pore size

100 Å

operating pH range

2-6

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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This Item
581514-U581513-U581502-U
Ascentis&#174; Si HPLC Column 5&#160;&#956;m particle size, L × I.D. 15&#160;cm × 2.1&#160;mm

581509-U

Ascentis® Si HPLC Column

Supelco

581514-U

Ascentis® Si HPLC Column

Ascentis&#174; Si HPLC Column 5&#160;&#956;m particle size, L × I.D. 25&#160;cm × 4.6&#160;mm

581513-U

Ascentis® Si HPLC Column

Ascentis&#174; Si HPLC Column 3&#160;&#956;m particle size, L × I.D. 15&#160;cm × 2.1&#160;mm

581502-U

Ascentis® Si HPLC Column

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

matrix active group

silica phase

matrix active group

silica phase

matrix active group

silica phase

matrix active group

silica phase

agency

suitable for USP L3

agency

suitable for USP L3

agency

suitable for USP L3

agency

suitable for USP L3

pore size

100 Å

pore size

100 Å

pore size

100 Å

pore size

100 Å

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)
The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 50(5), 703-709 (2009-06-30)
The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous
Deborah Benhaim et al.
Journal of chromatography. A, 1217(1), 65-74 (2009-11-27)
This study investigates lipophilicity determination by chromatographic measurements using the polar embedded Ascentis RP-Amide stationary phase. As a new generation of amide-functionalized silica stationary phase, the Ascentis RP-Amide column is evaluated as a possible substitution to the n-octanol/water partitioning system
Shigehisa Uchiyama et al.
Analytical chemistry, 80(9), 3285-3290 (2008-03-18)
A new method for the simultaneous determination of ozone and carbonyls in air using a two-bed cartridge system has been developed. Each bed consists of reagent-impregnated silica particles. The first contains trans-1,2-bis-(4-pyridyl) ethylene (BPE) while the second contains 2,4-dinitrophenylhydrazine (DNPH).
Paweł Kubica et al.
Journal of chromatography. A, 1289, 13-18 (2013-04-04)
Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach

Protocols

Amino Acid Analysis is the suitable tool for precise determination of protein quantities but also provides detailed information regarding the relative amino.

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