Skip to Content
MilliporeSigma
  • Enhancement of lomefloxacin Hcl ocular efficacy via niosomal encapsulation: in vitro characterization and in vivo evaluation.

Enhancement of lomefloxacin Hcl ocular efficacy via niosomal encapsulation: in vitro characterization and in vivo evaluation.

Journal of liposome research (2016-06-01)
Rawia M Khalil, Ghada A Abdelbary, Mona Basha, Ghada E A Awad, Hadeer A El-Hashemy
ABSTRACT

The aim of this study is to develop and evaluate niosomal dispersions loaded with the hydrophilic drug; lomefloxacin Hcl (LXN) for the management of ocular bacterial conjunctivitis. LXN-loaded niosomes were prepared by the thin film hydration method following a full factorial formulation design. Two independent variables were evaluated: the type of surfactant (X1) and the surfactant:cholesterol ratio (X2). The dependent variables comprised entrapment efficiency (EE%: Y1), particle size (PS: Y2) and zeta potential (ZP: Y3). The optimum formulation, N-LXN14 (Tw60: CH, 1:1), was spherical in shape and exhibited EE% of 68.41 ± 0.07, PS of 176.0 ± 0.98 and ZP of -40.70 ± 2.20 with a sustained release profile over 8 hours following the Higuchi model. N-LXN14 proved good physicochemical stability under refrigeration up to 3 months. Ocular irritancy test showed no signs of ocular toxicity, confirming the safety and suitability for ocular application. Microbiological evaluation of the antibacterial effect of N-LXN14 was conducted using the susceptibility test and through the induction of topical conjunctivitis by Staphylococcus aureus (S. aureus) followed by topical therapy. Susceptibility test manifested significantly higher percent inhibition of S. aureus and higher AUC

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Suplatast tosylate, ≥98% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 mL
Estimated to ship on March 22, 2025
$254.00
Sigma-Aldrich
Sorbitan monopalmitate
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 mL
Estimated to ship on March 22, 2025
$254.00
Sigma-Aldrich
Sorbitan monostearate, meets FCC analytical specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 mL
Estimated to ship on March 22, 2025
$254.00