A validated LC-MS/MS method for the determination of tolterodine and its metabolite in rat plasma and application to pharmacokinetic study.

A validated LC-MS/MS method for the determination of tolterodine and its metabolite in rat plasma and application to pharmacokinetic study.

Journal of pharmaceutical analysis (2013-12-01)

Rihana Parveen Shaik, Srinivasa Babu Puttagunta, Chandrasekhar Bannoth Kothapalli, Bahlul Zayed Sh Awen, B R Challa

ABSTRACT

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express RP amide (50 mm×4.6 mm, 2.7 μm) column with an isocratic mobile phase composed of 10 mM ammonium acetate and acetonitrile in the ratio of 20:80 (v/v), at a flow-rate of 0.5 mL/min. Tolterodine, tolterodine-d6, 5-hydroxy methyl tolterodine and 5-hydroxy methyl tolterodine-d14 were detected with proton adducts at

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565303-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm

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565305-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm

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565304-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm

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565306-U

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Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm

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565347-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 15 cm × 21.2 mm

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565371-U

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Ascentis^® RP-Amide Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm

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565370-U

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Ascentis^® RP-Amide Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

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565372-U

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Ascentis^® RP-Amide Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm, pkg of 2 ea

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565373-U

Supelco

Ascentis^® RP-Amide Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 2.1 mm

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565348-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm

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50779-U

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Ascentis^® Express RP-Amide, 5 μm Guard Cartridge, 5 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

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50751-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 5 cm × 3 mm

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50773-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm

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50753-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 10 cm × 3 mm

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50739-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm

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50759-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm

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50774-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

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50777-U

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Ascentis^® Express RP-Amide, 5 μm Guard Cartridge, 5 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

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50775-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 3 cm × 3 mm

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50737-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm

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50768-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm

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50758-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 3 mm

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 3 cm × 4.6 mm

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 2 cm × 2.1 mm

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565325-U

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Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

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565324-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

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50733-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 3 cm × 2.1 mm

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50734-U

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Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm

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50776-U

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Ascentis^® Express RP-Amide, 5 μm Guard Cartridge, 5 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea

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