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565348-U

Supelco

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 25 cm × 21.2 mm

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eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Quality Level

agency

suitable for USP L60

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

19.5% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 21.2 mm

surface area

450 m2/g

surface coverage

2.7 μmol/m2

impurities

<5 ppm metals

matrix

fully porous particle
silica gel high purity, spherical

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

100 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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This Item
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Supelco

565348-U

Ascentis® RP-Amide HPLC Column

Supelco

565344-U

Ascentis® RP-Amide HPLC Column

Ascentis&#174; RP-Amide HPLC Column 3&#160;&#956;m particle size, L × I.D. 10&#160;cm × 2.1&#160;mm

565301-U

Ascentis® RP-Amide HPLC Column

Ascentis&#174; RP-Amide HPLC Column 3&#160;&#956;m particle size, L × I.D. 15&#160;cm × 2.1&#160;mm

565302-U

Ascentis® RP-Amide HPLC Column

particle size

5 μm

particle size

5 μm

particle size

3 μm

particle size

3 μm

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

matrix

fully porous particle, silica gel high purity, spherical

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

pore size

100 Å

pore size

100 Å

pore size

100 Å

pore size

100 Å

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Features and Benefits

  • Excellent retention and peak shape for polar compounds
  • 100% aqueous compatibility
  • Ultra low bleed, LC-MS compatible
  • Unique selectivity

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Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express

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