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GC Analysis of Volatiles in White Wine on VOCOL® after SPME using a 75 μm Carboxen/PDMS Fiber

GC Analysis of Volatiles in White Wine on VOCOL® after SPME using a 75 μm Carboxen/PDMS Fiber suitable for GC, application for SPME

Materials

analytical column

Product No.
Description
Pricing

VOCOL® Capillary GC Column

L × I.D. 30 m × 0.25 mm, df 1.50 μm

SPME fiber

Product No.
Description
Pricing

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS)

df 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga

mobile phase component

Product No.
Description
Pricing

Methyl formate

analytical standard

related product

Product No.
Description
Pricing

SPME Fiber Holder

for use with manual sampling

SPME Fiber Holder

for use with CTC CombiPAL and Gerstel MPS2

standard

Product No.
Description
Pricing

Hexyl acetate

analytical standard

Octanoic acid

analytical standard

Ethyl butyrate

analytical standard

Isoamyl alcohol

≥98%, FG

2-Methyl-1-butanol

≥99%

Sulfur dioxide

≥99.9%

Ethyl hexanoate

analytical standard

Ethyl octanoate

analytical standard

2,3-Butanediol

analytical standard, mixture of racemic and meso forms

CONDITIONS

sample/matrix

white wine + 25% NaCl

SPME fiber

Carboxen/PDMS, 75 μm (57318)

extraction

10 min, 40 °C (headspace)

desorption process

3 min at 290 °C

column

VOCOL, 30 m × 0.25 mm I.D., 1.5 μm (24205-U)

oven

40 °C (2 min) to 220 °C at 10 °C/min

inj. temp.

290 °C

detector

GC-MS, quadrupole, m/z = 31-240

carrier gas

helium, 35 cm/sec

injection

splitless/split, closed 1 min

liner

0.75 mm liner

sample

white wine

Description

Analysis Note

In this application, SPME was used for the analysis of volatiles in white wine using a headspace method. The presence of aroma compounds in white wine is one of the most important attributes of its final quality. A Carboxen/PDMS fiber was inserted into the headspace of the vial for 10 minutes at 40 °C and analyzed by GC/MS. The thick film of the VOCOL GC column helps focus the volatile analytes.

Legal Information

VOCOL is a registered trademark of Merck KGaA, Darmstadt, Germany