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GC Analysis of Terpenes in Cannabis on Equity-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber

GC Analysis of Terpenes in Cannabis on Equity™-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber suitable for GC/MS, suitable for solid phase microextraction (SPME)

Materials

analytical column

Product No.
Description
Pricing

Equity-1 Capillary GC Column

L × I.D. 60 m × 0.25 mm, df 0.25 μm

SPME fiber

Product No.
Description
Pricing

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS)

needle size 23 ga, StableFlex, for use with autosampler

accessory

Product No.
Description
Pricing

Inlet Liner, Direct (SPME) Type, Straight Design (unpacked)

pkg of 1 ea

Headspace vial, screw top, rounded bottom (vial only)

volume 10 mL, clear glass vial, thread for 18, O.D. × H 22.5 mm × 46 mm, pkg of 100 ea

Magnetic Screw Cap for Headspace Vials, 18 mm thread

PTFE/silicone septum (white PTFE/blue silicone), septum thickness 1.5 mm, pkg of 100 ea

Molded Thermogreen® LB-2 Septa, with injection hole

diam. 11 mm, pkg of 50 ea

standard

Product No.
Description
Pricing

C7 - C40 Saturated Alkanes Standard

certified reference material, 1000 μg/mL each component in hexane

beta-Myrcene solution

certified reference material, 2000 μg/mL in hexane, ampule of 1 mL

Terpene Mix A

certified reference material, 2000 μg/mL each component in methanol

Terpene Mix B

certified reference material, 2000 μg/mL each component in methanol, ampule of 1 mL

CONDITIONS

sample/matrix

0.5 g dried cannabis in 10 mL headspace vial

SPME fiber

Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), 50/30 μm (57298-U)

extraction

20 min, headspace, 40 °C

desorption process

3 min, 270 °C

sample preparation

30 min equilibration at 40 °C prior to extraction

sample preparation

fiber postbake after extraction, 3 min at 270 °C

column

Equity-1, 60 m x 0.25 mm I.D., 0.25 μm (28047-U)

oven

60 °C (2 min), 5 °C/min to 275 °C (5 min)

inj. temp.

270 °C

detector

MSD

MSD interface

300 °C

carrier gas

helium, 1 mL/min constant flow

liner

0.75 mm ID, SPME

Description

Analysis Note

Terpenes are contained in the essential oils derived from plants, and often impart characteristic aromas to the plant and/or oil. Cannabis sativa (cannabis) contains more than 100 different terpenes and terpenoids, including mono, sesqui, di and tri, as well as other misc. compounds of terpenoid orgin. Terpene profile does not necessarily indicate geographic orgin of a cannabis sample, however it can be used in forensic applications to determine the common source of different samples. In addition, different cannabis strains have been developed which have distinct aromas and flavors, a result of the differing amounts of specific terpenes present. In this application, headspace-SPME combined with GC/MS was used to analyze some of the terpenes present in dried cannabis. Sample was obtained courtesy of Dr. Hari H. Singh, Program Director at the Chemistry & Physiological Systems Research Branch of the National Institute on Drug Abuse at the National Institute of Health. The exact strain of the sample was not known. Chromatographic separation was performed on an Equity-1 capillary GC column, and identification was done using retention indices and spectral library match.

Legal Information

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