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GC Analysis of FAMEs in Walnuts on SLB®-IL111

GC Analysis of FAMEs in Walnuts on SLB®-IL111 suitable for GC

Materials

analytical column

Product No.
Description
Pricing

SLB®-IL111 Capillary GC Column

L × I.D. 100 m × 0.25 mm, df 0.20 μm

related product

Product No.
Description
Pricing

Aldrich® single-neck round-bottom flask

capacity 50 mL, joint: ST/NS 24/40

Aldrich® modified Friedrichs condenser with SafetyBarb® tubing connectors

jacket O.D. 37 mm, Overall H 270 mm

Aldrich® modified Friedrichs condenser with SafetyBarb® tubing connectors

jacket O.D. 37 mm, Overall H 420 mm

Aldrich® modified Friedrichs condenser with SafetyBarb® tubing connectors

jacket O.D. 54 mm, Overall H 300 mm

BF3 - Methanol

10 % (w/w)

CONDITIONS

column

SLB-IL111, 100 m x 0.25 mm I.D., 0.20 μm (29647-U)

oven

168 °C

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

FID, 250 °C

injection

1 μL, split 100:1

liner

4 mm ID FocusLiner w/taper

sample

ground walnuts prepared for analysis per AOCS Official Method Ce 1k-09

Description

Analysis Note

This application illustrates the extraction and GC analysis of walnuts for fatty acids. Walnut is considered a significant plant source of alpha-linolenic acid (ALA). Both ALA and linoleic acid (LA) are the predominant essential fatty acids in humans (1). The samples were prepared using acid digestion /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (2). BHT was added as an antioxidant prior to extraction. All samples were concentrated to 1 mL prior to GC analysis. The FAME composition of vegetable oils is normally determined using AOCS Method Ce 1h-05, which designates the use of the a cyanopropylsilicone column (3). The SLB-IL111 is a highly polar column which provides alternate selecitivty and resolution of geometric positional isomers of fatty acid methyl esters. It can be used to complement the method designated column.

Legal Information

SLB is a registered trademark of Merck KGaA, Darmstadt, Germany