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HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 20 °C

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 20 °C application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53569-U

Ascentis® Express F5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

mobile phase component

Product No.
Description
Pricing

70221

Ammonium formate

eluent additive for LC-MS, LiChropur™, ≥99.0%

standard

Product No.
Description
Pricing

705993

3-epi-25-Hydroxyvitamin D3

98% (CP)

H-073

25-Hydroxyvitamin D2 solution

50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

H-083

25-Hydroxyvitamin D3 solution

100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

[A] 7 mM ammonium formate; [B] methanol (25:75)

flow rate

0.4 mL/min

pressure

5221 psi (245 bar)

column temp.

20 °C

detector

(UV, 265 nm, ESI(+), m/z 100 - 1000)

injection

1 μL

sample

300 mg/L in 25:75, water:methanol

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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