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HPLC Analysis of Acetaminophen, Caffeine, Chlorpheniramine, Ibuprofen, Naproxen, Phenylephrine, and Pseudoephedrine on Ascentis® Express C18

HPLC Analysis of Acetaminophen, Caffeine, Chlorpheniramine, Ibuprofen, Naproxen, Phenylephrine, and Pseudoephedrine on Ascentis® Express C18 application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

Standard

Product No.
Description
Pricing

Caffeine

Sigma Reference Standard, vial of 250 mg

Acetaminophen

analytical standard

Maleic acid

ReagentPlus®, ≥99% (HPLC)

Naproxen

meets USP testing specifications

Ibuprofen

≥98% (GC)

(−)-Pseudoephedrine

purum, ≥96.0% (sum of enantiomers, GC)

Chlorpheniramine Maleate

Pharmaceutical Secondary Standard; Certified Reference Material

Phenylephrine

European Pharmacopoeia (EP) Reference Standard

CONDITIONS

column

Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)

mobile phase

[A] 5 mM ammonium phosphate monobasic, pH 2.0 with phosphoric acid: acetonitrile (98:2); [B] acetonitrile;

gradient

0 to 68% B in 3 min; held at 68% B for 2 min

flow rate

0.4 mL/min

column temp.

35 °C

detector

UV, 210 nm

injection

1 μL

sample

100 μg/mL in 95:5, water: methanol

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of various over the counter drugs.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany