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HPLC Analysis of Aflatoxins in Wheat on Ascentis® Express C18 after SPE using Supel Tox AflaZea

HPLC Analysis of Aflatoxins in Wheat on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

related product

Product No.
Description
Pricing

Coors Buchner funnel

capacity 120 mL

standard

Product No.
Description
Pricing

Aflatoxin Mix 4 solution

analytical standard

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

25 g wheat meal spiked with pesticide standards

SPE tube/cartridge

SupelTox AflaZea, 6 mL (55314-U)

sample addition

2 mL

elution

collect eluate in 5 mL silanized culture tubes

eluate post-treatment

combine 200 μL of sample eluate and 880 μL of DI water in a 2 mL autosampler vial

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 μL/L nitric acid

flow rate

0.400 mL/min

pressure

4568 psi (315 bar)

column temp.

35 °C

detector

FLD 360 ex; 440 em

injection

20 μL

derivatization

via KOBRA electrochemical cell, 50 μA

Description

Analysis Note

Sample/matrix:
Weigh 25 grams of wheat meal into a 500 mL Erlenmeyer flask. Add 100 mL of 84:16 acetonitrile:water, seal, and place on an orbital shaker at med-high speed for 90 minutes. Filter the sample through a #2 qualitative filter. Spike sample at 2 mg/mL using analytical standards.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC