HPLC Analysis of Aspirin and Salicylic Acid on Ascentis® Express RP-Amide
CONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase
[A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA; (65:35, A:B)
flow rate
1 mL/min
pressure
2200 psi (152 bar)
column temp.
30 °C
detector
UV, 250 nm
injection
5 μL
sample
50 μg/mL in 75:25, water:acetonitrile
Description
Analysis Note
Salicylic acid is the primary metabolite of aspirin, and is likely responsible for its antiinflammatory properties via suppression of cyclooxygenase genes; it also is thought to suppress colonic carcinogenesis. Ascentis Express RP-Amide provides good retention and resolution at low pH by a combination of hydrophobic and H-bonding intereactions.
Legal Information
Ascentis is a registered trademark of Sigma-Aldrich Co. LLC