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HPLC Analysis of B-Trichothecenes on Ascentis® Express C18

HPLC Analysis of B-Trichothecenes on Ascentis® Express C18 application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

53814-U

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

Standard

Product No.
Description
Pricing

34134

B-Trichothecene mix, (DON, NIV, 3-AcDON, 15-AcDON)

~100 μg/mL in acetonitrile (each of DON, NIV, 3-AcDON and 15-AcDON), analytical standard

CONDITIONS

column

Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] water:acetonitrile:methanol (92:4:4); [B] acetonitrile

gradient

0% B held for 5 minutes, to 8% B in 3 minutes, 8% B held for 2 minutes

flow rate

0.8 mL/min

pressure

4061 psi (280 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

40 μL

sample

2 ppm each in water:methanol:acetonitrile (92:4:4)

Description

Analysis Note

Mycotoxins are toxic secondary metabolites produced by fungi. Those chemicals exist in food as a result of fungal infection of crops. Mycotoxins have strong resistance to decomposition and digestion, so they remain in the food chain in meat and dairy products. They also resist to temperature, such as cooking and freezing. Their effects on human and animal health include death, cancer, weakened immune systems and as allergens or irritants.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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