MilliporeSigma

HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express C18

HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

standard

Product No.
Description
Pricing

Acesulfame K

Aspartame

analytical standard

Benzoic acid

analytical standard

Caffeine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] 20 mM ammonium acetate, pH 4.7 with acetic acid: [B] acetonitrile; (90:10, A:B)

flow rate

0.6 mL/min

column temp.

40 °C

detector

UV, 214 nm

injection

1 μL

sample

100 - 500 μg/mL in 95:5, buffer: acetonitrile

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany