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HPLC Analysis of Cyclophosphamide Enantiomers on Astec® CHIROBIOTIC® T

HPLC Analysis of Cyclophosphamide Enantiomers on Astec® CHIROBIOTIC® T application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

Cyclophosphamide monohydrate

bulk package

CONDITIONS

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] ethanol; [C] THF; (90:5:5, A:B:C)

flow rate

0.75 mL/min

column temp.

23 °C

detector

UV, 205 nm

injection

5 μL

sample

1 mg/mL in methanol

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC