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HPLC Analysis of Ginsenoside Standards on Ascentis® Express C18

HPLC Analysis of Ginsenoside Standards on Ascentis® Express C18 application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Standard

Product No.
Description
Pricing

Ginsenoside-Rb1 from Panax ginseng (Korean ginseng) root

≥98% (HPLC), triterpenoid saponin

Ginsenoside-Rc from Panax ginseng (Korean ginseng) root

triterpenoid saponin, ≥98% (HPLC)

Ginsenoside Rg1

analytical standard

Ginsenoside Rf

analytical standard

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A]: water; [B]: acetonitrile

gradient

held at 25% for 1.5 min; 25 to 85% B in 12 min; held at 85% B for 1 min

flow rate

1.0 mL/min

pressure

3481 psi (240 bar)

column temp.

60 °C

detector

UV, 205 nm

injection

25 μL

sample

50 μg/mL in water:methanol (82:18)

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of ginseng.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany