HPLC Analysis of Ginsenoside Standards on Ascentis® Express C18
Materials
Analytical column
Product No.
Description
Pricing
Standard
CONDITIONS
column
Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase
[A]: water; [B]: acetonitrile
gradient
held at 25% for 1.5 min; 25 to 85% B in 12 min; held at 85% B for 1 min
flow rate
1.0 mL/min
pressure
3481 psi (240 bar)
column temp.
60 °C
detector
UV, 205 nm
injection
25 μL
sample
50 μg/mL in water:methanol (82:18)
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of ginseng.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany