MilliporeSigma

HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX

HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX application for HPLC

Materials

standard

Product No.
Description
Pricing

Hemoglobin from bovine blood

lyophilized powder

Hemoglobin from bovine blood

suitable for protease substrate, substrate powder

Hemoglobin from bovine blood

dried erythrocytes

Hemoglobin equine

lyophilized powder

Hemoglobin from bovine blood

lyophilized, 2×cryst., ≥90% (SDS-PAGE), powder

Hemoglobin from bovine blood

suitable for microbiology

CONDITIONS

column

Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)

mobile phase

[A] 10 mM Tris, pH 8.0 with acetic acid; [B] 10 mM Tris, 0.25 M KCl, pH 8.0 with acetic acid

gradient

0 to 32% B in 20 min. (linear)

flow rate

0.5 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

50 μg each Hb variants (A2, S and A0)

Description

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany