HPLC Analysis of Histidine Enantiomers on Astec^® CHIROBIOTIC^® T (23 °C)

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] 160 mM sodium phosphate, pH 4.5; [B] ethanol; (40:60, A:B)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 205 nm

injection

5 μL

sample

1 mg/mL in methanol