HPLC Analysis of Insulin (Species Variants) on Discovery® BIO Wide Pore C5
Materials
Analytical column
Product No.
Description
Pricing
CONDITIONS
column
Discovery BIO Wide Pore C5, 15 cm × 4.6 mm I.D., 5 μm particles (568422-U)
mobile phase
[A]: 71:29, (0.1% TFA in water):(0.1% TFA in acetonitrile);
[B]: 68:32, (0.1% TFA in water):(0.1% TFA in acetonitrile)
gradient
0-100% B in 30 min
flow rate
1 mL/min
column temp.
ambient
detector
UV, 215 nm
injection
5 μL 5 μL in 0.1% TFA containing 5ug each of three insulin variants
Description
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany