HPLC Analysis of Methadone Enantiomers on Astec^® CHIROBIOTIC^® V2

column

CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)

mobile phase

[A] methanol; [B] 20 mM ammonium formate; (95:5, A:B)

flow rate

1 mL/min

column temp.

20 °C

detector

UV, 205 nm

injection

2 μL

sample

methadone, 1 mg/mL in mobile phase