HPLC Analysis of Simple Underivatized Sugars on apHera^™ NH2

column

apHera NH2, 15 cm × 4.6 mm I.D., 5 μm particles (56401AST)

mobile phase

[A] water: [B] acetonitrile; (20:80, A:B)

flow rate

1.0 mL/min

column temp.

25 °C

detector

ELSD, 45 °C, 3.5 psi nitrogen

injection

10 μL

sample

500 μg/mL in 30:70, water: acetonitrile