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LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18

LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53822-U

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

standard

Product No.
Description
Pricing

H4014

25-Hydroxycholecalciferol

≥98% (HPLC)

17937

25-Hydroxyvitamin D2

≥95.0% (HPLC)

CONDITIONS

column

Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)

mobile phase

[A] 10mM ammonium formate in water; [B] 10mM ammonium formate in acetonitrile; (32:68, A:B)

flow rate

0.4 mL/min

pressure

1711 psi (118 bar)

column temp.

35 °C

detector

ESI(+)

injection

2 μL

sample

10 μg/mL in 25:75, water:methanol

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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