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LC/MS Analysis of Fluoroamphetamine Drugs of Abuse on Ascentis® Express HILIC

LC/MS Analysis of Fluoroamphetamine Drugs of Abuse on Ascentis® Express HILIC application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Standard

Product No.
Description
Pricing

4-Fluoroamphetamine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

(±)-2-Fluoroamphetamine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

(±)-4-Fluoromethamphetamine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

(±)-2-Fluoromethamphetamine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

mobile phase component

Product No.
Description
Pricing

Ammonium formate

eluent additive for LC-MS, LiChropur, ≥99.0%

CONDITIONS

column

Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)

gradient

5 mM ammonium formate in acetonitrile:water (90:10, v/v)

flow rate

0.4 mL/min

column temp.

35 °C

detector

TOF/MS

injection

2 μL

sample

300 ng/mL in acetonitrile

Description

Analysis Note

The fluoroamphetamines (FA) are a group of "designer" stimulant drugs in amphetamine family. The rapid LC/MS analysis of four members of the FA family on Ascentis Express HILIC is shown here. Cerilliant CRMs were used to ensure reliable MS identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany