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LC/MS Analysis of Polar Amino Acids on Ascentis® Si (HILIC Mode)

LC/MS Analysis of Polar Amino Acids on Ascentis® Si (HILIC Mode) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

L-Asparagine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

L-Threonine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Serine

European Pharmacopoeia (EP) Reference Standard

Aspartic acid

United States Pharmacopeia (USP) Reference Standard

Glutamine

United States Pharmacopeia (USP) Reference Standard

Glutamic acid

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] water; [C] acetonitrile

gradient

5% A constant; 10 to 16% B, 85 to 79% C in 20 min

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI(+)

injection

2 μL

sample

0.1 g/L each in mobile phase

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany