LC/MS Analysis of Cardiac Depressants on Ascentis^® Express Biphenyl

column

Ascentis Express Biphenyl, 10 cm x 3.0 mm, 2.7 μm particles (64066-U)

mobile phase

10mM ammonium formate (70:30 water:acetonitrile) SW pH 7.0 (not adjusted)

flow rate

0.6 mL/min

pressure

1363 psi (94 bar)

column temp.

35 °C

detector

MS ESI(+), TOF, XIC

injection

5 μL

sample

300 ng/mL in 85:15,  water:methanol