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EPA Method 547: HPLC Analysis of Glyphosate and Glyphosate Degradation Product (Aminomethyl)phosphonic Acid (AMPA) on SUPELCOSIL SAX1

EPA Method 547: HPLC Analysis of Glyphosate and Glyphosate Degradation Product (Aminomethyl)phosphonic Acid (AMPA) on SUPELCOSIL™ SAX1 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

SUPELCOSIL SAX1 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

mobile phase component

Product No.
Description
Pricing

Monobasic potassium phosphate

United States Pharmacopeia (USP) Reference Standard

standard

Product No.
Description
Pricing

Phthaldialdehyde

suitable for HPLC fluorimetric detection of amino acids, ≥99% (HPLC), powder or crystals

CONDITIONS

column

SUPELCOSIL SAX1, 25 cm x 4.6 mm I.D., 5 μm particles (59138)

mobile phase

[A] 5 mM monobasic potassium phosphate (pH 1.9 with phosphoric acid); [B] methanol; (A:B, 96:4)

flow rate

0.5 mL/min

column temp.

38 °C

detector

fluorescence, excitation 338 nm, emission 455 nm

detector

post column conditions for the oxidation solution (see EPA Method 547): flow rate  0.2 mL/min, temperature 38 °C, reactor volume 500 μL; (post column conditions for the derivatization solution (see EPA Method 547): flow rate 0.5 mL/min, ambient temperature, mixing device 500 μL mixing loop (0.5 mm))

injection

100 μL

sample

100 ng/mL in water

Description

Analysis Note

EPA Method 547 outlines the analysis of glyphosate in drinking water by direct aqueous injection HPLC, post column derivatization, and fluorescence detection. Anion exchange columns can be used for this method and have been shown to produce excellent results. A system is described here using a silica-based anion-exchange HPLC column (SUPELCOSIL SAX1) instead of a polymer-based column. Advantages of the silica-based column include compatibility with organic solvents and higher pressure stability. These, in turn, lead to increased column lifetime.

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC