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UHPLC Analysis of Barbiturates on Titan C18

UHPLC Analysis of Barbiturates on Titan C18 application for UHPLC

Materials

standard

Product No.
Description
Pricing

U0750

Uracil

≥99.0%

B0375

Barbital

B-006

Butalbital solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

P-008

Phenobarbital solution

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)

mobile phase

[A] 0.1% ammonium acetate (not adjusted); [B] acetonitrile; (70:30, A:B)

flow rate

0.4 mL/min

pressure

7540 psi (520 bar)

column temp.

35 °C

detector

UV, 230 nm

Description

Analysis Note

Analysis of a barbiturate mix using an MS-compatible mobile phase and a Titan C18 UHPLC column is shown here. Ultra high purity LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

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