Recommended Products
packaging
pkg of 1 vial
shipped in
ambient
storage temp.
−20°C
General description
The SygRNA synthetic RNA system recruits FnCas9 nuclease to specific double stranded DNA targets. The FnCas9 tracrRNA (trans-activating crRNA) binds to a FnCas9 crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides FnCas9 protein to crRNA-specified sites in double stranded DNA. SygRNA FnCas9 synthetic tracrRNA and crRNAs are optimized for use with FnCas9 protein and compatible with a variety of nucleic acid delivery and digestion methods. To purchase custom crRNAs click "Request Quotation for Custom crRNA Synthesis" at sigma.com/SygRNA
Application
Functional Genomics/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology
Features and Benefits
HPLC-purified SygRNA FnCas9 tracrRNA oligo is optimized for use with target-specific Francisella novicida Cas9 crRNA and FnCas9 protein for genome editing applications, large fragment molecular cloning, and programmable restriction digestion.
Physical form
Dry
Preparation Note
Resuspend SygRNA FnCas9 tracrRNA oligo in RNase-free Tris-containing buffer with a pH 8.0 or less.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Protocols
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service