D5282
Lambda DNA EcoR I Digest
for DNA electrophoresis
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.25
Pricing and availability is not currently available.
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form
liquid
usage
100 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s Lambda DNA EcoR I Digest contains 6 fragments from 3,530 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Components
Sigma′s Lambda DNA EcoR I Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Other Notes
Contains 6 fragments, 3,530–21,226 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.
Preparation Note
Bacteriophage lambda DNA was digested with EcoR I to completion, then purified by phenol extraction.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
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