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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
5C9.2, monoclonal
化学種の反応性
rat, mouse, human
テクニック
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
phosphorylation (pTyr701)
遺伝子情報
human ... STAT1(6772)
mouse ... Stat1(20846)
詳細
STATs (Signal-Transducing Activators of Transcription) are the principle substrates of JAK kinases, and mediators of cytokine signaling. STAT proteins have SH2 domains, and once phosphorylated by JAK kinases, STATs dimerize in a head-to-tail fashion, through their SH2 domains. STAT1 is a critical mediator of IFN signaling, STAT4 is specific to IL-12, STAT6 is activated primarily by IL-4 and IL-13, while STAT5 A and B appear to be most important to the function of growth hormone and prolactin. STAT3 is involved in multiple signaling pathways, and mice deficient in it are not viable. In addition to tyrosine phosphorylation, many STAT proteins are targets of Ser/Thr kinases, and those phosphorylation events potentiate STAT-induced transcription. The phosphorylation state of STATs can be monitored by phosphorylation state-specific antibodies.
特異性
This antibody recognizes phosphorylated STAT1 at tyrosine 701.
免疫原
Epitope: Phosphorylated Tyr701
KLH-conjugated linear peptide corresponding to the region around phosphorylated tyrosine 701.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
転写因子
転写因子
Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2 detects level of Phospho-Stat1 (Tyr701) & has been published & validated for use in WB.
品質
Routinely evaluated by western blot on EGF treated and untreated A431 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this lot was used to detect STAT1 phosphorylated at Tyr701 in EGF treated and untreated A431 cell lysates.
Western Blot Analysis: 0.5-2 µg/mL of this lot was used to detect STAT1 phosphorylated at Tyr701 in EGF treated and untreated A431 cell lysates.
ターゲットの説明
88-92 kDa
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS and 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8ºC from date of receipt.
アナリシスノート
Control
EGF treated and untreated A431 cell lysates, PDGF treated and untreated NIH/3T3 cell lysates, pervanadate treated and untreated PC12 cell lysates.
EGF treated and untreated A431 cell lysates, PDGF treated and untreated NIH/3T3 cell lysates, pervanadate treated and untreated PC12 cell lysates.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
05-1064:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
J E Darnell
Science (New York, N.Y.), 277(5332), 1630-1635 (1997-09-12)
STATs (signal transducers and activators of transcription) are a family of latent cytoplasmic proteins that are activated to participate in gene control when cells encounter various extracellular polypeptides. Biochemical and molecular genetic explorations have defined a single tyrosine phosphorylation site
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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