05-1406

Anti-Phospholipase A2 Antibody, clone CH-7

clone CH-7, from mouse

• 別名: Phospholipase A2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: CH-7, monoclonal
• 化学種の反応性: rat, human, mouse
• テクニック: ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG2b
• NCBIアクセッション番号: NM_000928.2
• UniProtアクセッション番号: P04054
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PLA2G10(8399)

詳細

詳細

Phospholipases hydrolyze phosphoglycerides to form smaller compounds which often serve as critical second messengers. Phospholipase A2 (PLA2) hydrolyzes phosphatidylcholine to produce lysophosphatidylcholine, a second messenger involved in cell proliferation, adhesion, T-cell activation, and smooth muscle cell contractility. PLA2 isoforms include membrane-associated, Ca2+-independent forms, cytosolic, Ca2+-dependent forms, and secretory forms. PLC catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate, to generate inositol (1,4,5) trisphosphate (IP3) and diacylglycerol (DAG), leading to Ca2+ release and PKC activation, respectively. At least four types of PLC have been identified. PLCβ, is activated by G-Protein-Coupled Receptors (GPCRs) and heterotrimeric G-Proteins. Mechanisms of PLCδ activation are unclear, but may also involve GPCRs. PLCγ, is activated by receptor tyrosine kinases, and appears essential for signaling by the PDGF Receptor. PLCε is a Guanine Nucleotide Exchange factor (GEF) for the small G-Protein Ras. PLD activity is up-regulated when cells are stimulated by hormones, growth factors or other extracellular signals. Two distinct PLDs, PLD1 and PLD2, have been identified. PLD hydrolyzes phosphatidylcholine to release phosphatidic acid, which is associated with a number of cellular responses including regulated secretion, mitogenesis and cytoskeletal changes.

特異性

This antibody recognizes PLA2.

免疫原

Recombinant cPLA2

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
脂質シグナル伝達

Anti-Phospholipase A2 Antibody, clone CH-7 detects level of Phospholipase A2 & has been published & validated for use in WB, ELISA, IH(P).

ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by the end user.

Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of PLA2 Monoclonal Antibody: Colon Cancer

Western Blot: A previous lot of this antibody was used at 1:500-1:1000; antibody recognizes all isoforms, the cPLA. Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa.

品質

Routinely evaluated by Western Blot on RAW 264 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PLA2 on 10 μg of RAW 264 lysates.

ターゲットの説明

Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa

関連事項

Replaces: MAB1668

物理的形状

Protein A purified

Format: Purified

Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6.

保管および安定性

Stable at 2-8°C for 1 year from date of receipt.

アナリシスノート

Control
RAW264 Cell Lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable