05-421

Anti-PP2A Antibody, C subunit, clone 1D6

clone 1D6, Upstate^®, from mouse

• 別名: Replication protein C, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, protein phosphatase 2, catalytic subunit, alpha isoform, serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 1D6, monoclonal
• 化学種の反応性: Saccharomyces cerevisiae, bovine, rabbit, rat, Xenopus, mouse, human
• メーカー／製品名: Upstate^®
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2bκ
• NCBIアクセッション番号: NP_002706
• UniProtアクセッション番号: P67775
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPP2CA(5515)

詳細

詳細

Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal downmodulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.

特異性

This antibody recognizes PP2A catalytic subunit, Mr 36 kDa.

免疫原

A 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A). Clone 1D6.

Epitope: a.a. 295-309

アプリケーション

Research Category
細胞シグナル伝達

ニューロサイエンス

Research Sub Category
神経伝達物質及び受容体

神経変性疾患

Detect PP2A using this Anti-PP2A Antibody, C subunit, clone 1D6 validated for use in IC, IP & WB.

Immunoprecipitation:
4 µg of a previous lot immunoprecipitated PP2A from 500 µg of A431 RIPA lysate. Note: This antibody has been reported not to block phosphatase activity.

Immunocytochemistry:
5-10 µg/mL of a previous lot of this antibody showed positive immunostaining for PP2A in A431 cells fixed for 10 minutes with 4% paraformaldehyde in PBS.

品質

Routinely evaluated by western blot on human A431 or murine 3T3/NIH RIPA cell lysates.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected PP2A in human A431 and previously in murine 3T3 RIPA cell lysates.

ターゲットの説明

36 kDa

物理的形状

Protein A chromatography

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15 M, NaCl, and 0.05% sodium azide. Frozen solution.

保管および安定性

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

アナリシスノート

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable