14-450-M

Cdk1/cyclin B Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.

• 別名: Active Cdk1/cyclin B, Cdk1 Protein
• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.26

特性

• 由来生物: human
• 品質水準: 100
• 形状: liquid
• シェルフライフ: 6 mo.
• 分子量: M_w 35 kDa (cdk1); M_w 75 kDa (cyclin B)
• メーカー／製品名: Upstate^®
• テクニック: activity assay: suitable (kinase)
• 溶解性: water: soluble
• NCBIアクセッション番号: NM_001786.2; NM_033379.2
• UniProtアクセッション番号: P06493
• 保管温度: −70°C
• 遺伝子情報: human ... CCNB1(891) , CDK2(891)

詳細

詳細

Research area: Apoptosis
Cyclin dependent kinase 1 (CDK1), a type of serine/threonine kinase, is a catalytic subunit of the M phase-promoting factor (MPF) complex.

生物化学的／生理学的作用

Cyclin B protein regulates mitosis in all eukaryotes. Cyclin B binds to cyclin dependent kinase 1 (CDK1), forming a complex that triggers the start of mitosis by phosphorylating specific proteins. Cyclin B regulates the activation, subcellular localization, and target selection of CDK1. Its destruction is essential for the completion of mitosis. Overexpression of human cyclin B1 has been linked to aggressive tumor behavior and is frequently observed in various cancers.
CDK1 plays a key role in driving the M-phase in both meiosis and mitosis. It becomes highly active at the start of the M-phase and becomes inactive at the end. CDK1 controls various important events in the cell, such as DNA replication, segregation, and repair, as well as mRNA transcription and cell morphogenesis. Additionally, it regulates ribosome assembly by targeting specific ribosomal proteins.

包装

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-450M when ordering the 250 µg size.

品質

routinely evaluated by phosphorylation of the Histone H1 substrate

物理的形状

Purified using Ni²⁺/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose^™ and Ni2+/NTA-agarose

保管および安定性

6 months at -70°C

その他情報

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法的情報

Sepharose is a trademark of Cytiva

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.