17-344
H2A.X Phosphorylation Assay Kit (Flow Cytometry)
The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X.
別名:
Assay for H2A.X, Flow Cytometry Assay Kit, H2A.X Phosphorylation Kit
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About This Item
UNSPSCコード:
12161503
eCl@ss:
32161000
NACRES:
NA.32
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品質水準
メーカー／製品名
Upstate®
テクニック
activity assay: suitable
flow cytometry: suitable
NCBIアクセッション番号
UniProtアクセッション番号
検出方法
fluorometric
輸送温度
dry ice
関連するカテゴリー
詳細
Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated histone H2A.X.
The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by the addition of the anti-phospho-Histone H2A.X, FITC conjugate. Cells are then scanned in a flow cytometer to quantitate the number of cells staining positive for phosphorylated Histone H2A.X.
The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by the addition of the anti-phospho-Histone H2A.X, FITC conjugate. Cells are then scanned in a flow cytometer to quantitate the number of cells staining positive for phosphorylated Histone H2A.X.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経変性疾患
神経変性疾患
包装
100 assays
構成
Anti-phospho H2A.X, FITC conjugate
Normal Mouse IgG, FITC conjugate (Cat.# 12-487)
16X Fixation Solution
10X Permeabilization Solution
10X Wash Solution
Normal Mouse IgG, FITC conjugate (Cat.# 12-487)
16X Fixation Solution
10X Permeabilization Solution
10X Wash Solution
品質
phosphorylation of Histone H2A.X
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
シグナルワード
Danger
危険有害性の分類
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
ターゲットの組織
Eyes,Central nervous system, Respiratory system
保管分類コード
3 - Flammable liquids
適用法令
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試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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