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Merck
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主要文書

安全性情報

217570

Sigma-Aldrich

Anti-CD3 Mouse mAb (UCHT1)

liquid, >95% (immunoglobulin, SDS-PAGE), clone UCHT1

別名:

Anti-CD3 Antibody, CD3 Detection Antibody, Mouse Anti-CD3

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.43

由来生物

mouse

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

UCHT1, monoclonal

アッセイ

>95% (immunoglobulin, SDS-PAGE)

形状

liquid

含みます

0.5 mg/mL gentamycin sulfate as preservative

化学種の反応性

human

メーカー/製品名

Calbiochem®

保管条件

OK to freeze

アイソタイプ

IgG1

輸送温度

wet ice

保管温度

2-8°C

ターゲットの翻訳後修飾

unmodified

詳細

Anti-CD3, mouse monoclonal, clone UCHT1, recognizes the ε-chain of the CD3 complex in human peripheral T cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.
Protein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.
Recognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.

免疫原

human thymocytes/Sezary T cells

アプリケーション

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see application references)

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

その他情報

Thibault, G. and Bardos, P. 1995. J. Immunol.154, 3814.
Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol.11, 329.
This antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 105 cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類コード

10 - Combustible liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

217570-100UG:
217570-1MG:
217570-0MG:
217570-UG:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Wenmin Ji et al.
American journal of cancer research, 13(11), 5218-5235 (2023-12-07)
This research is dedicated to investigating the mechanism of programmed cell death ligand 1 (PD-L1) and tumor protein 53 target gene 1 (TP53TG1) in immune regulation of colon cancer (CC). Expressions of TP53TG1, PD-L1 and signal transducers and activators of
Mengting Chen et al.
International journal of biological sciences, 19(16), 5160-5173 (2023-11-06)
Rosacea is a common inflammatory skin disorder mediated by the dysregulation of both keratinocytes and T cells. Here, we report that aquaporin 3 (AQP3), a channel protein that mediates the transport of water/glycerol, was highly expressed in the epidermis and
Xiaofei Gao et al.
Frontiers in immunology, 13, 799331-799331 (2022-03-18)
Trace element iron affects T cell biology, but the knowledge about the role of iron in regulating Treg cell expansion is limited. Treg cells play an important role in keeping peripheral T cell tolerance, increasing Treg cell expansion is a
Zijun Wang et al.
The Journal of clinical investigation, 130(7), 3717-3733 (2020-03-20)
T follicular helper (Tfh) cells are indispensable for the formation of germinal center (GC) reactions, whereas T follicular regulatory (Tfr) cells inhibit Tfh-mediated GC responses. Aberrant activation of Tfh cells contributes substantially to the pathogenesis of autoimmune diseases, such as
Javier Robles-Valero et al.
The EMBO journal, 40(22), e108125-e108125 (2021-10-08)
Mutations in VAV1, a gene that encodes a multifunctional protein important for lymphocytes, are found at different frequencies in peripheral T-cell lymphoma (PTCL), non-small cell lung cancer, and other tumors. However, their pathobiological significance remains unsettled. After cataloguing 51 cancer-associated

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