C7831

モノクローナル抗CD28抗体 マウス宿主抗体

clone CD28.2, purified from hybridoma cell culture

• 別名: モノクローナル抗CD28抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: CD28.2, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen 44 kDa
• 化学種の反応性: human, rhesus monkey
• テクニック: flow cytometry: 5 μL using 1 × 10⁶ cells; immunocytochemistry: suitable; immunohistochemistry (frozen sections): suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P10747
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CD28(940)

詳細

詳細

CD28 (Cluster of differentiation 28) is a glycosylated membrane protein. The protein exists as a homodimer. CD28 is encoded by the gene mapped to human chromosome 2q33. The encoded protein is a co-stimulatory receptor expressed on naive T-cells. CD28 is characterized with a short cytoplasmic tail and two proline-rich motifs.

特異性

Recognizes the CD28 (44 kDa) human cell surface disulfide-linked, homodimeric glycoprotein. The antibody reacts with human CD28-transfected murine T-cell hybridoma, PHA-activated blasts and IL-6 dependent plasmacytoma lines. It cross reacts with rhesus monkey PBL. The antibody blocks CD28/B7 (CD80) mediated cell-cell adhesion. It binds to the CDR domain in a CDR1 dependent manner. It enhances CD3 monoclonal antibody-induced T-cell proliferation. May be used in adhesion and co-stimulation studies.
5th Workshop: code no. CD28.05.

免疫原

human CD28 transfected murine T cell hybridoma.

アプリケーション

Monoclonal Anti-CD28 antibody produced in mouse is suitable for the following applications:

• Flow cytometry at a dilution of 5μL using 1 × 106 cells
• Immunocytochemistry
• Immunohistochemistry (frozen sections)

The antibody has been used for development of an assay to identify small molecules antagonists of CD28/CD86 binding.

生物化学的／生理学的作用

CD28 (Cluster of differentiation 28) is a protein expressed on T-cells that provide co-stimulatory signals required for T-cell activation, survival and differentiation into effector T cells. CD28 is also expressed on multiple myeloma (MM) cells and is a key mediator of MM survival and apoptotic resistance. CD28 pro-survival signaling is dependent upon downstream activation of phosphatidylinositol 3-kinase (PI3K)/AKT (RAC-alpha serine/threonine-protein kinase), inactivation of the transcription factor FoxO3a and decreased expression of the pro-apoptotic molecule BIM. CD28 plays an important role as a mediator of MM survival during stress and can be targeted to overcome chemotherapy resistance. Inflammatory CD28^- T-cells are found to be accumulated in livers of patients with primary sclerosing cholangitis (PSC) and are localized around bile ducts. Autoantibodies against CD28 are found in patients with autoimmune and atopic diseases.

ターゲットの説明

CD28 is expressed on thymocytes, peripheral lineage cell subsets, activated B cells and plasma cells. The antigen is involved in adhesion between T and B lymphocytes through the B7/BB-1 ligand expressed on activated B cells. CD28 co-stimulates T-cells activated via the CD3 T-cell receptor and CD2 pathways and induces their proliferation.

物理的形状

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable