MABF175E

Anti-CD3 (human), redFluor^® 710, clone UCHT1 Antibody

clone UCHT1, from mouse, redFluor^® 710

• 別名: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD antigen CD3e, CD3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: redFluor^® 710
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: UCHT1, monoclonal
• 化学種の反応性: human
• テクニック: flow cytometry: suitable
• UniProtアクセッション番号: P07766
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CD3E(916)

詳細

詳細

The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activationinduced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein.

免疫原

Immunogen information for this antibody is proprietary information.

アプリケーション

Research Category
炎症及び免疫

Research Sub Category
免疫グロブリン及び免疫

This mouse monoclonal Anti-CD3 Antibody (human), redFluor^® 710, clone UCHT1, Cat. No. MABF1758 is validated for use in Flow Cytometry, for the detection of CD3.

品質

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD3 in one million human peripheral blood lymphocytes.

物理的形状

Purified by size-exclusion chromatography

Purified Mouse monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

redFluor is a registered trademark of Tonbo Biotechnologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable