219364

Cathepsin B, Human Liver

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

• 別名: Cathepsin B, Human Liver
• CAS番号: 9047-22-7
• Enzyme Commission number: 3.4.22.1 (BRENDA, IUBMB)
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 由来生物: human liver
• 品質水準: 100
• アッセイ: >95% (SDS-PAGE)
• 形状: liquid
• 比活性: ≥50 units/mg protein
• 精製方法: affinity chromatography
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• テクニック: activity assay: suitable
• pH範囲: 3.5—6.0
• 適合性: suitable for molecular biology
• アプリケーション: life science and biopharma
• 輸送温度: wet ice
• 保管温度: −70°C
• 遺伝子情報: human ... CTSB(1508)

詳細

詳細

Research area: Cell Signaling

Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.

Optimal pH: 3.5-6.0.

アプリケーション

Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.

生物化学的／生理学的作用

Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.

物理的形状

In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.

調製ノート

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

再構成

Following initial thaw, aliquot and freeze (-70°C).

その他情報

Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable