SRP0289

Cathepsin B Active human

recombinant, expressed in FreeStyle^™ 293-F cells, ≥90% (SDS-PAGE)

• 別名: APP secretase, CPSB
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: human
• リコンビナント: expressed in FreeStyle^™ 293-F cells
• アッセイ: ≥90% (SDS-PAGE)
• 形状: aqueous solution
• 比活性: ≥2228 pmol/min-μg
• 分子量: 43 kDa
• 濃度: >1 mg/mL
• テクニック: activity assay: suitable
• 溶解性: water: soluble
• 適合性: suitable for molecular biology
• NCBIアクセッション番号: NM_001908
• アプリケーション: life science and biopharma
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... CTSB(1508)

詳細

詳細

Research area: Cell signaling. Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide(amino acids 1-16) + Cathepsin B (amino acids 18-339), with C-terminal HIS tag, MW = 43 kDa, expressed in FreeStyle 293-F cells. Cathepsin B is a cysteine protease, found in the lysosome of normal cells and tissues. Cathepsin B active human is encoded by the gene CTSB, located on human chromosome 8p23.1.

アプリケーション

Active human cathepsin B is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin B has been used in a study to assess maturation through proenzyme activation and proteolytic processing. Active human cathepsin B has also been used in a study to prepare a catalytically active version from its precursor expressed in Escherichia coli in the form of inclusion bodies.

Cathepsin B Active human has been used as a supplement in serum free media for the treatment of MDA-MB-231 cell line. It has also been used as a component in low pH buffer for collagen II and complement 3 processing.

生物化学的／生理学的作用

Cathepsin B Active human primarily is a preproenzyme, glycosylated on rough endoplasmic reticulum. Processing of procathepsin B to a 31 kDa, single chain active form occurs in the lysosome. It is further processed to double chain form with a 25/26 kDa heavy chain and 5 kDa heavy chain in lysosome. Cathepsin B, functions as an exopeptidase at low pH. At higher pH in endosomes or neutral pH, cathepsin B functions as endopeptidase. Cathepsin B expression is upregulated in malignant tumors and facilitates tumor invasion. Cathepsin B might reduce the production of amyloid-β peptide associated with Alzheimerβs disease. It also plays a key role in neuroprotective functions.

単位の定義

1 unitは、37°Cで1分間に1 pmolの基質を切断する酵素量として定義されています。

物理的形状

Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。

法的情報

FreeStyle is a trademark of Invitrogen Corp.

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H315,H319,H360D
• 注意書き: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable