SRP0145

PRMT5/MEP50 Active human

recombinant, expressed in FreeStyle^™ 293-F cells, ≥60% (SDS-PAGE)

• 別名: Arginine methyltransferase 5, HMT1 hnRNP methyltransferase-like 5(HRMT1L5), ICln-binding protein (IBP72) 72 kDa, Jak-binding protein 1 (JBP1), shk1 kinase-binding protein 1 homolog (SKB1)
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in FreeStyle^™ 293-F cells
• アッセイ: ≥60% (SDS-PAGE)
• フォーム: aqueous solution
• 分子量: 37.5 kDa (MEP50); 73 kDa (PRMT5)
• 包装: pkg of 20 μg
• メーカー／製品名: Sigma-Aldrich
• 保管条件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• テクニック: inhibition assay: suitable
• 溶解性: water: soluble
• NCBIアクセッション番号: NM_006109 (PRMT5); NM_024102 (MEP50)
• UniProtアクセッション番号: O14744
• アプリケーション: life science and biopharma
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... PRMT5(10419)

詳細

詳細

Research area: Cell signaling. Protein arginine methyltransferase 5 (PRMT5) and WD repeat-containing protein (WDR77) or methylosome protein- 50 (MEP-50) are part of the methylosome. PRMT5 is a type II PRMT enzyme. The gene encoding it is localized to human chromosome 14q11.2.Complex of human PRMT5 (GenBank Accession No. NM_006109), amino acid 2-end, with N-terminal FLAG^®-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), amino acid 2-end, with N-terminal His tag, MW=37.5 kDa, expressed in FreeStyle 293-F cells.

アプリケーション

酵素キネティクスの研究をはじめ、インヒビターのスクリーニングや選択性プロファイリングにもご利用いただけます。

Recombinant PRMT5/MEP50 has been used in the detection of arginine methylation in in vitro methyltransferase assay.

生物化学的／生理学的作用

Methylosome protein 50 (MEP50) binds to the oncogenic protein arginine methyltransferase 5 (PRMT5) and forms a complex called methylosome. This complex is involved in mono- and symmetric dimethylation of arginine, which is necessary for various biological processes. MEP50 acts as a coactivator for androgen receptor (AR) and estrogen receptor (ER). PRMT5 also methylates histones and other proteins involved in genome organization, transcription, stem cells, primordial germ cells, differentiation, the cell cycle, and spliceosome assembly. The two proteins have been associated with cancer tumorigenesis and are marked as potential oncogenes.A boost in MEP50 levels is observed in several human cancers such asastrocytoma, ovarian cancer, lung cancer, glioblastoma, breast cancer,testicular tumor, and prostate cancer.

単位の定義

1ユニットは、37°Cで1分あたり基質1 pmolのメチル化に必要な酵素量と定義されています。

物理的形状

Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 μg/ml FLAG^® peptide, and 20% glycerol.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注した酵素液は希釈せずそのまま-70°Cで保存してください。注:酵素は凍結融解の繰り返しに対して非常に不安定です。

法的情報

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FreeStyle is a trademark of Invitrogen Corp.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable