SRP0291

Cathepsin L Active human

recombinant, expressed in FreeStyle^™ 293-F cells, ≥90% (SDS-PAGE)

• 別名: CATL, Major excreted protein (MEP)
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: human
• リコンビナント: expressed in FreeStyle^™ 293-F cells
• アッセイ: ≥90% (SDS-PAGE)
• 形状: aqueous solution
• 比活性: ≥3900 pmol/min-μg
• 分子量: 36 kDa
• テクニック: inhibition assay: suitable
• 適合性: suitable for molecular biology
• NCBIアクセッション番号: NM_001912
• アプリケーション: life science and biopharma
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... CTSL(1514)

詳細

詳細

Research area: Cell Signaling

Cathepsin L is a papain-like cysteine protease and belongs to the Clan A, Family C1. It is composed of L domain of α-helix and an R domain of β-sheet in the spatial structure.

Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.

アプリケーション

Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used:

• to determine that N-acyl and N-sulfonyloxazolidine-2,4-diones are pseudo-irreversible inhibitors of serine proteases
• to investigate the role of cathepsin B and L activity in the serum during the human aging process.
• in inhibitory activity assay
• to study the role of CTSL in COVID-19 infection

生物化学的／生理学的作用

Cathepsin L is a cysteine protease, involved primarily in protein breakdown in the lysosome. Cathepsin L degrades nuclear transcription factors and may affect cell cycle regulation. Cathepsin L is also involved in the immune system, specifically in degrading the invariant chain during major histocompatibility complex (MHC) class II processing, a crucial step in antigen presentation. Its expression in the thymus has been demonstrated to be vital for the development of natural killer cells. Additionally, Cathepsin L contributes to recycling processes during axon outgrowth and synapse formation in the developing postnatal central nervous system. Proteases are associated with the development and progression of cancer. Therefore, cathepsin L may be a potential therapeutic target in cancer treatment.

単位の定義

1 unitは、37°Cで1分間に1 pmolの基質を切断する酵素量として定義されています。

物理的形状

Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。

法的情報

FreeStyle is a trademark of Invitrogen Corp.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable